Insecticidal Activity of Kaffir Lime (Citrus hystrix) against Rice Weevil (Sitophylus oryzae)
DOI:
https://doi.org/10.58915/aset.v3i.584Abstract
The aim of this study is to analyze the potential use of kaffir lime leaves and peel extract as bioinsecticides against rice weevils. The assessment of bioinsecticidal activity was studied through contact activity, repellency, antifeedant, and progeny and growth development tests. Contact activity tests resulted in increased adult mortality with increased extract concentrations from 10 to 50 mg/L after 24 hours. The leaf extract (LC50=18 mg/L) showed a higher mortality rate compared to fruit peel extracts (LC50=54 mg/L), with 100 % and 60 % mortality, respectively. The leaf extracts with a mean repellency of 68 % significantly repelled rice weevils compared to the 42 % repellency of fruit peel extracts. The leaf extract was classified in repellency class IV, while the peel extract was in repellency class II. With the use of Kaffir lime leaf extracts, F1 adults were completely suppressed at 20 mg/L concentration of leaf extract with a 100 % inhibition rate.
Keywords:
Insecticidal activity, rice weevil, kaffir lime, kaffir lime leaves, kaffir lime peelReferences
Khani, M., Awang, R. M., Omar, D., Rahmani, M., & Rezazadeh, S. Tropical medicinal plant extracts against rice weevil, Sitophilus oryzae L. Journal of Medicinal Plants Research, vol 5, issue 2 (2011) pp. 259–265.
Ali, A., Ahmad, F., Biondi, A., Wang, Y., & Desneux, N. Potential for using Datura alba leaf extracts against two major stored grain pests, the khapra beetle Trogoderma granarium and the rice weevil Sitophillus oryzae. Journal of Pest Science, vol 85, issue 3 (2012) pp. 359–366.
Buatone, S., & Indrapichate, K. Protective effects of mintweed, kitchen mint and kaffir lime leaf extracts against rice weevils, Sitophilus oryzae L., in stored, milled rice. International Journal of Agriculture Sciences, vol 3, issue 3 (2011) p. 133.
Mya, M. M., Aye, Y. Y., Oo, A. W., & Saxena, R. K. Effect of Citrus hystrix DC Leaves Ethanol Extract on Larvae of Aedes aegypti. Journal of Biological Engineering Research and Review, vol 2, issue 2 (2015).
Rajkumar, A., Balamurugan, P., & Prasad, M. P. Comparative Phytochemical Analysis of Rutaceae Family (Citrus Species) Extracts. International Journal of Scientific Research, vol 3, issue 4 (2012) pp. 148–150.
Gomathi, S., & Rathinam, K. M. S. Evaluation of repellency and antifeedant activities of Saraca asoca and Terminalia arjuna Bark extracts against Sitophilus oryzae. Evaluation, vol 2, issue 6 (2017).
McDonald, L. L., Guy, R. H., & Speirs, R. D. Preliminary evaluation of new candidate materials as toxicants, repellents, and attractants against stored-product insects, US Agricultural Research Service, vol 882, (1970).
Abdelgaleil, S. A., Mohamed, M. I., Shawir, M. S., & Abou-Taleb, H. K. Chemical composition, insecticidal and biochemical effects of essential oils of different plant species from Northern Egypt on the rice weevil, Sitophilus oryzae L. Journal of Pest Science, vol 89, issue 1 (2016) pp. 219–229.
Kerdchoechuen, O., Laohakunjit, N., Singkornard, S., & Matta, F. B. Essential oils from six herbal plants for biocontrol of the maize weevil. HortScience, vol 45, issue 4 (2010) pp. 592–598.
Finney, D. J., & Tattersfield, F. The adjustment for a natural response rate in probit analysis. Annals of Applied Biology, vol 36, issue 2 (1952) pp. 187-195
Tapondjou, A. L., Adler, C., Fontem, D. A., Bouda, H., & Reichmuth, C. Bioactivities of cymol and essential oils of Cupressus sempervirens and Eucalyptus saligna against Sitophilus zeamais Motschulsky and Tribolium confusum du Val. Journal of Stored Products Research, vol 41, issue 1 (2005) pp. 91–102.
Maung Mya, M., Zar Aung, Z., That Nwe, C., Win Oo, A., Myat Htay, T., Thaung, S., & Naung Maung Maung, Y. Larvicidal, Ovicidal and repellent effect of Citrus hystrix DC (Kaffir lime) fruit, peel and internal materials extracts on Aedes aegypti mosquitoes. Journal of Biological Engineering Research and Review, vol 4, issue 1 (2017) pp. 34–43.
Abdullah, S. S. S., & Othman, M. K. I. Potential of Kaffir Lime (Citrus hystrix) Peel Essential Oil as a Cockroach Repellent. In Sustainable Technologies for the Management of Agricultural Wastes, (2018) pp. 131–141.
Md Othman, S., Hassan, M., Nahar, L., Basar, N., Jamil, S., Sarker, S., … Sarker, S. D. Essential Oils from the Malaysian Citrus (Rutaceae) Medicinal Plants. Medicines, vol 3, issue 2 (2016) p.13.
Loh, F. S., Awang, R. M., Omar, D., & Rahmani, M. Insecticidal properties of Citrus hystrix DC leaves essential oil against Spodoptera litura fabricius. Journal of Medicinal Plants Research, vol 5, issue 16 (2011) pp. 3739–3744.
