Evaluation of Fructose Concentration on Xanthan Gum Bioproduction Using Black Soldier Fly Larvae as a Sustainable Nitrogen Source

Ahmad Ramli Rashidi; Nurul Izani Binti M Razali; Muhammad Ikman Ishak

doi:10.58915/aset.v5i2.3280

Authors

Ahmad Ramli Rashidi Universiti Teknologi MARA
Nurul Izani Binti M Razali Universiti Teknologi MARA
Muhammad Ikman Ishak Universiti Malaysia Perlis

DOI:

https://doi.org/10.58915/aset.v5i2.3280

Keywords:

Black Soldier Fly Larvae (BSFL) hydrolysate, Xanthomonas campestris ATCC 13951, Fructose concentration, Xanthan gum yield, Sustainable fermentation

Abstract

Xanthan gum is a valuable polysaccharide used in the food and pharmaceutical industries, but its conventional production relies on costly, unsustainable glucose and commercial nitrogen sources. This study evaluates the use of Black Soldier Fly Larvae (BSFL) as an alternative nitrogen source and investigates the effect of different fructose concentrations on xanthan gum production by Xanthomonas campestris ATCC 13951. Experiments were conducted at 30°C and 150 rpm with varying fructose concentrations, and biomass yield (CDW) and xanthan production were analyzed after 24 hours. The results indicated that a fructose concentration of 30 g/L produced the highest xanthan yield (3.80 g/L), while higher concentrations led to metabolic inhibition. This indicates that optimizing fructose levels is essential for maximizing yield. This study also highlights the potential of BSFL as a sustainable alternative to nitrogen, contributing to cost-effective and environmentally friendly bioprocessing. These findings support the integration of waste-based resources in microbial fermentation, aligning with Sustainable Development Goal 12 (Responsible Consumption and Production) by promoting circular economy principles through waste valorization. Future research should systematically investigate the synergistic effects of critical fermentation parameters to optimize xanthan yield and quality.

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