Green Synthesis of Bio-ZnO Nanoparticles via Aloe Vera Powder-Assisted Sol–Gel Method with Antibacterial Properties

Authors

  • Muhamad Fikri Shohur Kolej Vocational Kerian
  • Zawati Harun Kolej Vocational Kerian & Universiti Tun Hussein Onn Malaysia
  • Muhamad Zaini Yunos Kolej Vocational Kerian
  • Mohd Riduan Jamalludin Universiti Malaysia Perlis
  • Siti Khadijah Hubadillah Universiti Utara Malaysia
  • Nurul Syazwani Mansor Kolej Vocational Kerian

DOI:

https://doi.org/10.58915/aset.v5i1.3206

Keywords:

Bio-ZnO NPs, Aloe vera powder, Green Synthesis, Sol-gel method

Abstract

This study presents the green synthesis of zinc oxide nanoparticles (bio-ZnO NPs) using Aloe vera powder as a natural reducing and stabilizing agent through the sol–gel method. The use of Aloe vera powder eliminates the need for toxic chemicals and simplifies the synthesis process, thereby promoting sustainability in nanomaterial fabrication. The obtained bio-ZnO NPs were characterized by UV–Vis spectroscopy, FTIR, XRD, FESEM, TEM, and EDX analyses. Results confirmed the successful formation of wurtzite hexagonal ZnO with uniform nanoscale morphology. The optical band gap was found to decrease slightly with increasing Aloe vera concentration, indicating the influence of phytochemical compounds on crystal growth and electronic structure. Antibacterial activity against Escherichia coli demonstrated significant inhibition zones, suggesting strong biological functionality of the synthesized nanoparticles. This work highlights the effectiveness of Aloe vera powder as a sustainable and low-cost precursor for producing high-quality ZnO nanoparticles with potential applications in environmental and antimicrobial technologies.

References

[1] Hendrawati, T. Y. Journal of engineering science and technology. Journal of Engineering Science and Technology, vol 10 (2015) pp.47–59.

[2] Sumesh, K. R., Kanthavel, K. Journal of polymers and the environment. Journal of Polymers and the Environment, vol 27 (2019) pp.2189–2200.

[3] Yusof, K. N., Alias, S. S., Harun, Z., Basri, H., Azhar, F. H. Chemistryselect. ChemistrySelect, vol 3 (2018) pp.8881–8885.

[4] Senthilkumar, S. R., Sivakumar, T. International journal of pharmacy and pharmaceutical sciences. International Journal of Pharmacy and Pharmaceutical Sciences, vol 6 (2014) pp.461–465.

[5] Hussin, R., Seng, G. H., Zulkiflee, N. S., Harun, Z. Zno/tio₂ thin films for photocatalytic application. AIP Conference Proceedings, vol 2068 (2019) pp.020096.

[6] Ainuddin, A. R., Kalidasan, K., Kamdi, Z., Ibrahim, S. A., Hussin, R., Junaid, T. M. The effect of zinc oxide nanostructure on the antibacterial activity. AIP Conference Proceedings, vol 2068 (2019) pp.020094.

[7] Azhar, F. H., Harun, Z., Yunos, M. Z., Ahmad, A., Mohd Hajar, S. H., Akhair, M., Ahmad, R. A. R., Ibrahim, S. A. Malaysian journal of fundamental and applied sciences. Malaysian Journal of Fundamental and Applied Sciences, vol 14 (2018) pp.397–402.

[8] Harun, Z., Shohur, M. F., Jamalludin, M. R., Yunos, M. Z., Basri, H. Hydrophilicity effect of rice husk silica on mixed matrix PSF membrane properties. Jurnal Teknologi (Sciences & Engineering), vol 70 (2014) pp.15–18.

[9] Essien, E. R., Atasie, V. N., Okeafor, A. O., Nwude, D. O. Biogenic synthesis of magnesium oxide nanoparticles using manihot esculenta (Crantz) leaf extract. International Nano Letters, vol 10 (2020) pp.43–48.

[10] Rasli, N. I., Basri, H., Harun, Z. Zinc oxide from aloe vera extract: Two-level factorial screening of biosynthesis parameters. Heliyon, vol 6 (2020) pp.e03156.

[11] Ishwarya, R., Vaseeharan, B., Kalyani, S., Banumathi, B., Govindarajan, M., Alharbi, N. S., Kadaikunnan, S., Mohammed, N. A., Khaled, J. M., Benelli, G. Facile green synthesis of zinc oxide nanoparticles using ulva lactuca seaweed extract and evaluation of their photocatalytic, antibiofilm and insecticidal activity. Journal of Photochemistry and Photobiology B: Biology, vol 178 (2018) pp.249–258.

[12] Perveen, R., Shujaat, S., Qureshi, Z., Nawaz, S., Khan, M. I., Iqbal, M. Green versus sol–gel synthesis of ZnO nanoparticles and antimicrobial activity evaluation against panel of pathogens. Journal of Materials Research and Technology, vol 9 (2020) pp.7817–7827.

[13] Mirzaei, H., Darroudi, M. Zinc oxide nanoparticles: Biological synthesis and biomedical applications. Ceramics International, vol 43 (2017) pp.907–914.

[14] Bhuyan, T., Mishra, K., Khanuja, M., Prasad, R., Varma, A. Green synthesis of zinc oxide nanoparticles and their characterization. Materials Science in Semiconductor Processing, vol 32 (2015) pp.55–61.

[15] Anju, T. R., Parvathy, S., Veettil, M. V., Rosemary, J., Ansalna, T. H., Shahzabanu, M. M., Devika, S. Green synthesis of silver nanoparticles from aloe vera leaf extract and its antimicrobial activity. Materials Today: Proceedings, vol 43 (2021) pp.3956–3960.

[16] Maensiri, S., Laokul, P., Klinkaewnarong, J., Phokha, S., Promarak, V., Seraphin, S. Indium oxide (in₂o₃) nanoparticles using aloe vera plant extract: Synthesis and optical properties. Optoelectronics and Advanced Materials – Rapid Communications, vol 2 (2008) pp.161–165.

[17] Ganapathi Rao, K., Ashok, C., Venkateswara Rao, K., Shilpa Chakra, C., Tambur, P. Green synthesis of tio₂ nanoparticles using aloe vera extract. International Journal of Advanced Research in Physical Science, vol 2 (2015) pp.28–34.

[18] Ahmad, R. A. R., Harun, Z., Azhar, F. H., Hussin, R., Zin, M. F. M., Sazali, N., Bahri, S. S., Jamaluddin, M. R., Misdan, N., Kamdi, Z. Polymer mixed membrane with microflower tio₂ as additive for photocatalyst in organic compound. Materials Today: Proceedings, vol 46 (2021) pp.2122–2130.

[19] Nalini, S. P. K., Vijayaraghavan, K. Green synthesis of silver and gold nanoparticles using aloe vera gel and determining its antimicrobial properties on nanoparticle impregnated cotton fabric. Journal of Nanotechnology Research, vol 2 (2020) pp.42–50.

[20] Gandhi, R. R., Sundrarajan, M. Green synthesis of tin oxide nanoparticles by aloe vera: Structural, optical and antibacterial properties. Journal of Nanoelectronics and Optoelectronics, vol 8 (2013) pp.240–249.

[21] Jayaseelan, C., Abdul Rahuman, A., Vishnu Kirthi, A., Marimuthu, S., Santhoshkumar, T., Bagavan, A., Gaurav, K., Karthik, L., Bhaskara Rao, K. V. Novel microbial route to synthesize ZnO nanoparticles using Aeromonas hydrophila and their activity against pathogenic bacteria and fungi. Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy, vol 90 (2012) pp.78–84.

[22] Sangeetha, G., Rajeshwari, S., Venckatesh, R. Green synthesis of zinc oxide nanoparticles by Aloe barbadensis miller leaf extract: Structure and optical properties. Materials Research Bulletin, vol 46, issue 12 (2011) pp.2560–2566.

[23] Zhang, G., Zhou, M., Xu, Z., Jiang, C., Shen, C., Meng, Q. Guanidyl-functionalized graphene/polysulfone mixed matrix ultrafiltration membrane with superior permselective, antifouling and antibacterial properties for water treatment. Journal of Colloid and Interface Science, vol 540 (2019) pp.295–305.

[24] Chan, Y. Y., Pang, Y. L., Lim, S., Chong, W. C. Facile green synthesis of ZnO nanoparticles using natural-based materials: Properties, mechanism, surface modification and application. Journal of Environmental Chemical Engineering, vol 9 (2021) pp.105417.

[25] Song, Y., Yang, F., Ma, M., Kang, Y., Hui, A., Quan, Z., Wang, A. Green synthesized se–zno/attapulgite nanocomposites using aloe vera leaf extract: Characterization, antibacterial and antioxidant activities. LWT, vol 165 (2022) pp.113762.

[26] Veisi, P., Seyed Dorraji, M. S., Vatanpour, V., Rasoulifard, M. H. Dimensional effect of zno-g-c₃n₄ heterostructures on hydrophilic and anti-fouling properties of the PVDF/PAN composite membrane: Dye rejection. Journal of Environmental Chemical Engineering, vol 11 (2023).

Downloads

Published

2026-06-02

How to Cite

Shohur, M. F., Harun, Z., Yunos, M. Z., Jamalludin, M. R., Hubadillah, S. K., & Mansor, N. S. (2026). Green Synthesis of Bio-ZnO Nanoparticles via Aloe Vera Powder-Assisted Sol–Gel Method with Antibacterial Properties. Advanced and Sustainable Technologies (ASET), 5(1), 211–222. https://doi.org/10.58915/aset.v5i1.3206

Issue

Section

Articles

Similar Articles

<< < 1 2 3 4 

You may also start an advanced similarity search for this article.