Performance of electrochemical amperometric sensor based on annealed and non-annealed PANI-Ag-Pt nanocomposite thin films for E. coli detection

Authors

  • Huda Abdullah Department of Electrical, Electronic and System Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, 43650 Bangi, Malaysia
  • Norshafadzila Mohammad Naim Department of Electrical, Electronic and System Engineering, Faculty of Engineering and Built Environment, Universiti Kebangsaan Malaysia, 43650 Bangi, Malaysia
  • Aidil Abdul Hamid School of Biosciences and Biotechnology, Faculty of Science and Technology, Universiti Kebangsaan Malaysia, 43650 Bangi, Malaysia
  • Noraziah Mohamad Zin Center of Diagnostic, Therapeutic and Investigative Studies, Faculty of Health Sciences, Universiti Kebangsaan Malaysia, 50300 Kuala Lumpur, Malaysia
  • Mohd Hafiz Dzarfan Othman Advanced Membrane Technology Research Centre (AMTEC), School of Chemical and Energy Engineering, Universiti Teknologi Malaysia, 81310 UTM Johor Bahru, Johor, Malaysia
  • Yap Wing Fen Department of Physics, Faculty of Science, Universiti Putra Malaysia, 43400 UPM Serdang, Selangor, Malaysia
  • Brian Yuliarto Faculty of Industrial Technology, Bandung Institute of Technology, Kota Bandung, Jawa Barat 40132, Indonesia

Abstract

Herein, we report the fabrication of polyaniline with Ag-Pt alloy nanocomposite thin films based amperometric E. coli sensor. The effects of annealing at 300oC during the synthesis of PANI-Ag-Pt nanocomposite thin films are studied. XRD, AFM, FESEM and FTIR spectroscopy analyses are conducted to characterize the films. Sensor performance is investigated by the I-V characteristic of PANI-Ag-Pt films with and without the presence of E. coli. From XRD analysis, annealed films have larger crystallite sizes than non-annealed films. AFM results indicate that annealed films have lower surface roughness but larger grain size than non-annealed films. FESEM images show Ag-Pt nanorods with sizes of 200-300 nm for non-annealed films and Ag-Pt nanocubes with sizes around 100-550 nm for annealed films. The composition of PANI-Ag0.2-Pt0.8 nanocomposite thin film has higher conductivity and performed the maximum sensitivity upon E. coli presence. The sensitivity of annealed PANI-Ag-Pt films is higher than non-annealed PANI-Ag-Pt films.

Keywords:

Polyaniline, Ag-Pt, thin films, Annealing, E. coli, Amperometric sensor

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Published

24-04-2025

How to Cite

[1]
Huda Abdullah, “Performance of electrochemical amperometric sensor based on annealed and non-annealed PANI-Ag-Pt nanocomposite thin films for E. coli detection”, IJNeaM, vol. 18, no. 2, pp. 204–212, Apr. 2025.

Issue

Vol. 18 No. 2 (2025): April

Section

Articles